Investigation of chitosan nanoparticles durability in combination with antioxidant-antibacterial fraction extracted from Lactobacillus casei and possible increase of antibacterial activity of the fraction in hybrid nanoparticle

Objective(s): This study considered the combination of chitosan nanoparticles with antioxidant-antibacterial fraction extracted from Lactobacillus casei and investigation of possible increasing of antibacterial activity of the fraction in hybrid nanoparticle and the effect of the fraction on the stability of chitosan nanoparticles. Methods: Extraction of Antioxidant antibacterial material from Lactobacillus casei supernatant was done by thin layer chromatography fractionation. For determination of antioxidant and antibacterial activity of fraction, DPPH (2,2-diphenyl-1-picrylhydrazyl) assay and Minimum Inhibition Concentration (MIC) by micro-well dilution method was used, respectively. For chitosan nanoparticles (Cs NPs) formation, the ionic gelation method was used and the ratio of Tripolyphosphate pentasodium (TPP): chitosan was optimized. For Antioxidant fraction loaded chitosan nanoparticles, the fraction is physically incorporated into the chitosan nanoparticles. Particle morphology was monitored by Scanning Electron Microscopy (SEM). Results: One polar fraction by Rf = 0.03 has a strong antibacterial activity that shows terpenoids characterization. Chitosan nanoparticle loaded antioxidant-antibacterial material has longer life span while compare to Cs-NPs alone. The antioxidant-antibacterial material was released relatively slowly from the CS NPs. the antibacterial trait of the fraction was increased about 8-16 times after combination with Cs NPs. Conclusions: Combination of Cs NPs with antioxidant-antibacterail fraction isolated from Lactobacillus casei increase the Cs NPs stability and antibacterial activity of the fraction was enhanced considerably, also.